Acid Solutions  (PDF)		Flammable Liquids  (PDF)
Acutely Toxic Chemicals  (PDF)		Formaldehyde  (PDF)
Carcinogens  (PDF)		Oxidizing Chemicals  (PDF)
Compressed Gases  (PDF)		Pyrophoric Chemicals  (PDF)
Corrosive Chemicals  (PDF)		Reproductive Toxins  (PDF)
Decaborane  (PDF)		Water Reactive Chemicals  (PDF)
Drugs  (PDF)

 

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ACID SOLUTIONS
STANDARD OPERATING PROCEDURE

HAZARDOUS CHEMICALS/CLASS OF HAZARDOUS CHEMICALS

• Acids: acetic, hydrochloric, nitric, phosphoric, sulfuric, phosphoric

HAZARD DESCRIPTION

• Topical exposure to acids may result in burns on the skin. Potential exposure occurs when handling aqueous acid solutions.

PROTECTION PROCESS

• Good laboratory technique
• Appropriate shielding through use of personal protective equipment
• Use of fume hood when dealing with concentrated (fuming) acids

PERSONAL PROTECTIVE EQUIPMENT

• Safety goggles (Wear chemical safety goggles when using small quantities or safety glasses or chemical safety goggles with face shield when using large quantities.)

• Nitrile, PVC, or neoprene gloves

• Rubber, neoprene, or PVC apron (when using large quantities and splash potential exists)

ENGINEERING/VENTILATION CONTROLS

• Use concentrated acids in fume hood.

• A safety shower and eyewash must be available and accessible when working with corrosive liquids.

SPECIAL HANDLING PROCEDURES AND STORAGE REQUIREMENTS

• Store mineral acids together, separate from oxidizing agents and organic materials.

• Store acetic acid and other organic acids with the combustible organic liquids.

SPILL AND ACCIDENT PROCEDURES

• Skin exposure: Rinse affected skin with plenty of water while removing contaminated clothing and shoes. Rinse for at least 15 minutes. Seek medical attention.

• Eye exposure: Splashes may cause tissue destruction. Wash eyes for at least 15 minutes, lifting the upper and lower eyelids occasionally. Seek medical attention immediately.

• Small spills:. Cover spill with sodium carbonate or bicarbonate. When reaction stops pickup with damp sponge or paper towels. (Do not attempt cleanup if you feel unsure of your ability to do so or if you perceive the risk to be greater than normal laboratory operations.)

• Large Spills: Notify others in area of spill. Turn off ignition sources in area. Evacuate area and post doors to spill area. Call Office of Environmental Health & Safety 292-1284 or University Police 911 (this is a 24 hour service). Remain on the scene, but at a safe distance, to receive and direct safety personnel when they arrive. Restrict persons from area of spill or leak until cleanup is complete. Remain in area in safe location to assist EH&S with response.

WASTE DISPOSAL

• Diluted solutions may be poured down the sink accompanied by copious amounts of water.
• Old, concentrated solutions should be disposed of through EH&S.

SPECIAL APPROVAL REQUIRED

• No

DECONTAMINATION

• Use sodium bicarbonate and water.

DESIGNATED AREA

• No

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ACUTELY TOXIC CHEMICALS
STANDARD OPERATING PROCEDURE

HAZARDOUS CHEMICALS/CLASS OF HAZARDOUS CHEMICALS

• Toxic chemicals are defined as those with an LD50 value for oral exposure in rats of 50-500 mg/kg, for skin contact in rabbits of 200-100 mg/kg, or for inhalation exposure in rats of 200-2000 ppm/air.
• Highly toxic chemicals are defined as those with an LD50 value for oral exposure in rats of less than 50500 mg/kg, for skin contact in rabbits of less than 200100 mg/kg, or for inhalation exposure in rats of less than 2002000 ppm/air.

HAZARD DESCRIPTION

• Acutely toxic chemicals can cause severe illness and sometimes death.

PROTECTION PROCESS

• Good laboratory technique
• Appropriate shielding through use of personal protective equipment
• Use of fume hood
• Availability of eye wash station and safety shower

PERSONAL PROTECTIVE EQUIPMENT

• Safety glasses/goggles (Wear chemical safety goggles when using small quantities or safety glasses or chemical safety goggles with face shield when using large quantities or when a splash potential exists.)
• Gloves should be worn when handling acutely toxic chemicals. Disposable latex or nitrile gloves provide adequate protection against accidental hand contact with small quantities of most laboratory chemicals. Lab workers should contact OEHS for advice on chemical resistant gloves when direct or prolonged contact with hazardous chemicals is anticipated.
• Lab coats, closed toed shoes and long sleeved clothing should be worn when handling corrosive materials.
• Additional protective clothing should be worn if the possibility of skin contact is likely.

ENGINEERING/VENTILATION CONTROLS

• Manipulation of acutely toxic chemicals should be carried out in a fume hood whenever possible.
• Under certain conditions, acutely toxic chemicals can be used in a Biological Safety Cabinet. The biological safety cabinet is designed to remove the acutely toxic chemicals before the air is discharged into the environment. Acutely toxic chemicals that are volatile must not be used in a biological safety cabinet unless the cabinet is vented to the outdoors.
• Certain acutely toxic chemicals must be handled in a glove box rather than a fume hood. The Division of Environmental Health and Safety (2-1284) or the Principal Investigator will determine if this is required.
• For small quantities of acutely toxic chemicals, portable shields, which provide protection to all laboratory occupants, are acceptable.

• A safety shower and eyewash must be available and accessible when working with acutely toxic chemicals.

SPECIAL HANDLING PROCEDURES AND STORAGE REQUIREMENTS

• All acutely toxic chemicals must be clearly labeled with the correct chemical name.
• Do not store acutely toxic materials on high cabinets or shelves.
• Acutely toxic chemicals must be stored in a designated area.
• The room sign where acutely toxic chemicals are stored must contain a "Designated Areas Within" identifier.

SPILL AND ACCIDENT PROCEDURES

• Before beginning work with acutely toxic chemicals, develop emergency procedures which address response actions to fires, explosions, spills, injury to staff, or the development of signs and symptoms of overexposure. The procedures should address as a minimum the following:

• Who to contact: (University police, and Office of Environmental Health and Safety,
• Principal investigator of the laboratory including evening phone number)
• The location of all safety equipment (showers, spill clean up supplies, eye wash, fire extinguishers, etc.)
• The method used to alert personnel in nearby areas of potential hazards
• Special first aid treatment required by the type of corrosive material(s) handled in the laboratory

• Anticipate spills by having clean up equipment on hand. The appropriate clean up supplies can be determined by consulting the material safety data sheet. This should occur prior to the use of any acutely toxic chemical.
• In the event of a spill, all personnel in the area should be alerted.
• Do not attempt to handle a spill of acutely toxic chemicals. Vacate the laboratory immediately and call for assistance (Division of Environmental Health & Safety 292-1284 or 911).
• Remain on the scene, but at a safe distance, to receive and direct safety personnel when they arrive.

WASTE DISPOSAL

• All materials contaminated with acutely toxic chemicals should be disposed of as a hazardous waste.
• Wherever possible, attempt to design research in a manner that reduces the quantity of waste generated.
• Questions regarding waste pick up should be directed to the Office of Environmental Health and Safety. This office can also assist you in minimizing waste generation.

SPECIAL APPROVAL REQUIRED

• The College of Pharmacy Safety Committee must approve work with acutely toxic chemicals.
• You should notify the Office of Environmental Health and Safety prior to the initial use of acutely toxic substances. Use the form in Appendix F for this purpose. Notification is also required following significant changes in procedures or the quantity of materials used.

DECONTAMINATION

Personnel:

• Wash hands and arms with soap and water immediately after handling acutely toxic chemicals.

Area:

• Decontamination procedures vary depending on the material being handled. The toxicity of some materials can be neutralized with other reagents. All surfaces should be wiped with the appropriate cleaning agent following dispensing or handling.
• Waste materials generated should be treated as a hazardous waste.

Equipment:

• Decontaminate glassware, vacuum pumps, etc. before removing them from the designated area.

DESIGNATED AREA

• All locations within the laboratory where acutely toxic chemicals are handled should be demarcated with designated area caution tape (available from OEHS, the Cell Center, or Chemistry stockroom) and/or posted with designated area caution signs. This includes all fume hoods and bench tops where the acutely toxic chemicals are handled.
• Where feasible acutely toxic chemicals should be manipulated over plastic-backed disposable paper work surfaces. These disposable work surfaces minimize work area contamination and simplify clean up.
• The room sign for the laboratory must contain a "Designated Areas Within" identifier.

 

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CARCINOGENS
STANDARD OPERATING PROCEDURE

HAZARDOUS CHEMICALS/CLASS OF HAZARDOUS CHEMICALS

• A carcinogen commonly describes any agent that can initiate or speed the development of malignant or potentially malignant tumors, malignant neoplastic proliferation of cells, or cells that possess such material. A list of some carcinogenic materials is found in Appendix ??.

HAZARD DESCRIPTION

• Carcinogens hazards can cause cancer.

PROTECTION PROCESS

• Good laboratory technique
• Appropriate shielding through use of personal protective equipment
• Use of fume hood
• Availability of eye wash station and safety shower

PERSONAL PROTECTIVE EQUIPMENT

• Safety glasses/goggles (Wear chemical safety goggles when using small quantities or safety glasses or chemical safety goggles with face shield when using large quantities or when a splash potential exists.)
• Gloves should be worn when handling carcinogens. Disposable latex or nitrile gloves provide adequate protection against accidental hand contact with small quantities of most laboratory chemicals. Lab workers should contact OEHS for advice on chemical resistant gloves when direct or prolonged contact with hazardous chemicals is anticipated.
• Lab coats, closed toed shoes and long sleeved clothing should be worn when handling carcinogens.
• Additional protective clothing should be worn if the possibility of skin contact is likely.

ENGINEERING/VENTILATION CONTROLS

• Manipulation of carcinogens should be carried out in a fume hood.
• Under certain conditions, carcinogens can be used in a biological safety cabinet. The biological safety cabinet is designed to remove the carcinogens before the air is discharged into the environment. Carcinogens that are volatile must not be used in a biological safety cabinet unless the cabinet is vented to the outdoors.
• Certain carcinogens must be handled in a glove box rather than a fume hood. The Division of Environmental Health and Safety or the Principal Investigator will determine if this is required.
• For small quantities of carcinogens, portable shields, which provide protection to all laboratory occupants, are acceptable.
• A safety shower and eyewash must be available and accessible when working with carcinogens.

SPECIAL HANDLING PROCEDURES AND STORAGE REQUIREMENTS

• Carcinogens must be stored in a designated area.

SPILL AND ACCIDENT PROCEDURES

• Before beginning work with carcinogens, develop emergency procedures which address response actions to accidental exposure from fires, explosions, or spills. The procedures should address as a minimum the following:

• Who to contact: (University police, and Office of Environmental Health and Safety,
• Principal investigator of the laboratory including evening phone number)
• The location of all safety equipment (showers, spill clean up supplies, eye wash, fire extinguishers, etc.)
• The method used to alert personnel in nearby areas of potential hazards
• Special first aid treatment required by the type of corrosive material(s) handled in the laboratory

• Anticipate spills by having clean up equipment on hand. The appropriate clean up supplies can be determined by consulting the material safety data sheet. This should occur prior to the use of any carcinogens.

• In the event of a spill, all personnel in the area should be alerted.
• Do not attempt to handle a large spill of carcinogens. Vacate the laboratory immediately and call for assistance (Division of Environmental Health & Safety 292-1284 or 911).
• Remain on the scene, but at a safe distance, to receive and direct safety personnel when they arrive.

WASTE DISPOSAL

• All materials contaminated with carcinogens should be disposed of as a hazardous waste.
• Wherever possible, attempt to design research in a manner that reduces the quantity of waste generated.
• Questions regarding waste pick up should be directed to the Office of Environmental Health and Safety.
• This office can also assist you in minimizing waste generation.

SPECIAL APPROVAL REQUIRED

• You should notify the Office of Environmental Health and Safety prior to the initial use of carcinogens.
• Notification is also required following significant changes in procedures or the quantity of materials used.

DECONTAMINATION

Personnel:

• Wash hands and arms with soap and water immediately after handling carcinogens.

Area:

• Decontamination procedures vary depending on the material being handled. The toxicity of some materials can be neutralized with other reagents. All surfaces should be wiped with the appropriate cleaning agent following dispensing or handling.
• Waste materials generated should be treated as hazardous waste.

Equipment:

• Decontaminate glassware or equipment before removing them from the designated area.

DESIGNATED AREA

• All locations within the laboratory where carcinogens are handled should be demarcated with designated area caution tape (available from OHES 292-1284) and/or posted with designated area caution signs. This includes all fume hoods and bench tops where the carcinogens are handled.
• Where feasible, carcinogens should be manipulated over plastic-backed disposable paper work surfaces. These disposable work surfaces minimize work area contamination and simplify clean up.
• The room sign for the laboratory must contain a "Designated Areas Within" identifier.

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COMPRESSED GASSES
STANDARD OPERATING PROCEDURE

HAZARDOUS CHEMICALS/CLASS OF HAZARDOUS CHEMICALS

• Cylinders containing compressed gasses.

HAZARD DESCRIPTION

• Cylinders of compressed gases must be handled as high energy sources. They can become a projectile if the cylinder valve is dislodged.

PROTECTION PROCESS

• Good laboratory technique

PERSONAL PROTECTIVE EQUIPMENT

• Not applicable to compressed gasses in general – may be needed for specific gasses.

ENGINEERING/VENTILATION CONTROLS

• Not applicable to compressed gasses in general – may be needed for specific gasses.

SPECIAL HANDLING PROCEDURES AND STORAGE REQUIREMENTS

• Cylinders should be stored in an upright position and secured to a wall or laboratory bench through the use of chains or straps.
• Cylinder caps should remain on the cylinder at all times unless a regulator is in place.
• Cylinders should be stored in areas where they will not become overheated. Avoid storage near radiators, areas in direct sunlight, steam pipes and heat releasing equipment such as sterilizers.
• Transport compressed gas cylinders on equipment designed for this function.
• Never carry or "walk" cylinders by hand.

SPILL AND ACCIDENT PROCEDURES

• Not applicable to compressed gasses in general – may be needed for specific gasses.

WASTE DISPOSAL

• All empty or partially filled compressed gas cylinders should be returned to the supplier. If the supplier does not accept empty or partially filled cylinders, contact the Office of Environmental Health and Safety concerning disposal.

SPECIAL APPROVAL REQUIRED

• Not applicable to compressed gasses in general – may be needed for specific gasses.

DECONTAMINATION

• Not applicable to compressed gasses in general – may be needed for specific gasses.

DESIGNATED AREA

• Not applicable to compressed gasses in general – may be needed for specific gasses.

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CORROSIVE CHEMICALS - ACIDS, BASES AND DEHYDRATING AGENTS
STANDARD OPERATING PROCEDURE

HAZARDOUS CHEMICALS/CLASS OF HAZARDOUD CHEMICALS

• Chemicals that are highly corrosive to steel.
• The major classes of corrosives include strong acids, bases, and dehydrating agents.

HAZARD DESCRIPTION

• Corrosive chemicals cause visible destruction or permanent changes in human skin tissue at the site of contact.

PROTECTION PROCESS

• Good laboratory technique
• Appropriate shielding through use of personal protective equipment
• Use of fume hood
• Availability of eye wash station and safety shower

PERSONAL PROTECTIVE EQUIPMENT

• Safety glasses/goggles (Wear chemical safety goggles when using small quantities or wafety glasses or chemical safety goggles with face shield when using large quantities or when a splash potential exists.)
• Gloves should be worn when handling corrosive chemicals. Disposable latex or nitrile gloves provide adequate protection against accidental hand contact with small quantities of most laboratory chemicals. Lab workers should contact OEHS for advice on chemical resistant gloves.
• Lab coats, closed toed shoes and long sleeved clothing should be worn when handling corrosive materials.
• Additional protective clothing should be worn if the possibility of skin contact is likely.

ENGINEERING/VENTILATION CONTROLS

• Manipulation of corrosive substances should be carried out in a fume hood if corrosive vapor production is anticipated.

• A safety shower and eyewash must be available and accessible when working with corrosive liquids.

SPECIAL HANDLING PROCEDURES AND STORAGE REQUIREMENTS

• Segregate the various types of corrosives. Separate acids and bases. Liquids and solids should also be separated.
• Specially designed corrosion resistant cabinets should be used for the storage of large quantities of corrosive materials.
• Store corrosives on plastic trays.
• All corrosive chemicals must be clearly labeled with the correct chemical name.
• Do not store corrosive materials on high cabinets or shelves.

SPILL AND ACCIDENT PROCEDURES

• Before beginning work with large amounts of corrosive chemicals, develop emergency procedures which address response actions to fires, explosions, spills, injury to staff, or the development of signs and symptoms of overexposure. The procedures should address as a minimum the following:

• Who to contact: (University police, and Office of Environmental Health and Safety,
• Principal investigator of the laboratory including evening phone number)
• The location of all safety equipment (showers, spill clean up supplies, eye wash, fire extinguishers, etc.)
• The method used to alert personnel in nearby areas of potential hazards
• Special first aid treatment required by the type of corrosive material(s) handled in the laboratory

• Anticipate spills by having clean up equipment on hand. The appropriate clean up supplies can be determined by consulting the material safety data sheet. This should occur prior to the use of any corrosive chemical. Corrosive spill controls neutralize the hazardous nature of the spilled material. Acids and bases require different types of spill control materials.
• In the event of a spill all personnel in the area should be alerted.
• If the incident involves Hydrofluoric acid (HF), seek immediate medical attention.
• If there is any doubt about the severity of the injury, seek immediate medical attention.
• Do not attempt to handle a large spill of corrosive materials. Vacate the laboratory immediately and call for assistance (Division of Environmental Health & Safety 292-1284 or 911).
• Remain on the scene, but at a safe distance, to receive and direct safety personnel when they arrive.

WASTE DISPOSAL

• Most corrosive materials are hazardous wastes. Questions regarding waste disposal should be directed to the Office of Environmental Health and Safety.

SPECIAL APPROVAL REQUIRED

• No

DECONTAMINATION

Personnel:

• Immediately flush contaminated area with copious amounts of water after contact with corrosive materials.
• Remove any jewelry to facilitate removal of chemicals.
• If a delayed response is noted, report immediately for medical attention. Be prepared to detail what chemicals were involved.

Area:

• Decontamination procedures vary depending on the material being handled. The corrosivity of some materials can be neutralized with other reagents. Special neutralizing agents should be on hand to decontaminate areas.

DESIGNATED AREA

Not applicable

 

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DECABORANE
STANDARD OPERATING PROCEDURE

HAZARDOUS CHEMICALS/CLASS OF HAZARDOUS CHEMICALS

• Decaborane, boron hydride (B10H14), Nido-decaborane(14), tetradecahydrodecaborane

HAZARD DESCRIPTION

• Flammable
• Highly toxic
• Very toxic by inhalation
• Readily absorbed through skin

PROTECTION PROCESS

• Good laboratory technique
• Appropriate shielding through use of personal protective equipment
• Non-sparking tools
• Use in fume hood at all times

PERSONAL PROTECTIVE EQUIPMENT

• Safety goggles
• Self-contained breathing apparatus
• Rubber boots
• Heavy rubber (chemical resistant) gloves
• Lab coats, closed toed shoes and long sleeved

ENGINEERING/VENTILATION CONTROLS

• A safety shower and eyewash must be available and accessible when working with decaborane
• A fume hood should be used

SPECIAL HANDLING PROCEDURES AND STORAGE REQUIREMENTS

• Keep storage to the minimum needed for research
• Keep away from water as decaborane reacts with water to liberate flammable and/or explosive gas
• Store in a cool, dry place

SPILL AND ACCIDENT PROCEDURES

• Anticipate spills by having the appropriate clean up equipment on hand. The appropriate clean up supplies can be determined by consulting the material safety data sheet. This should occur prior to the use of decaborane
• In the event of a spill all personnel in the area should be alerted. Turn off all sources of ignition. Vacate the laboratory immediately and call for assistance: Office of Environmental Health & Safety 292-1284 or 911 (This is a 24 hour service).
• Remain on the scene, but at a safe distance, to receive and direct safety personnel when they arrive.
• In case of contact, immediately flush eyes or skin with copious amounts of water for at least 15 minutes
• Remove contaminated clothing and shoes
• If swallowed, wash out mouth with water provided person is conscious
• If inhaled, remove to fresh air. If not breathing, give artificial respiration

WASTE DISPOSAL

• All waste material must be stored in labeled containers
• Call Office of Environmental health & Safety (292-1284) for pickup
• Do not pour solutions or solid decaborane down the sink

SPECIAL APPROVAL REQUIRED

• No

DECONTAMINATION

• Cover with sand or soda ash and immediately seek advice from Environmental Health and Safety

DESIGNATED AREA

• Yes

 

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DRUGS
STANDARD OPERATING PROCEDURE

HAZARDOUS CHEMICALS/CLASS OF HAZARDOUS CHEMICALS

• For purposes of this SOP and the College of Pharmacy Chemical Hygiene Plan, drugs are chemicals that are used or are under investigation for use in humans and/or other animals or are chemicals that are used in in vitro experiments with organs or tissue preparations.

HAZARD DESCRIPTION

• Ingestion of large amounts of some drugs can cause illness or even death.

PROTECTION PROCESS

• Good laboratory technique
• Appropriate shielding through use of personal protective equipment

PERSONAL PROTECTIVE EQUIPMENT

• Latex gloves for more hazardous compounds

ENGINEERING/VENTILATION CONTROLS

• Not normally needed

SPECIAL HANDLING PROCEDURES AND STORAGE REQUIREMENTS

• Not normally needed

SPILL AND ACCIDENT PROCEDURES

• For skin exposure, rinse affected skin with plenty of water

• For spills, clean affected area thoroughly

WASTE DISPOSAL

• Diluted solutions may be poured down the sink

SPECIAL APPROVAL REQUIRED

• No

DECONTAMINATION

• Not normally needed

DESIGNATED AREA

• No

 

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FLAMMABLE LIQUIDS
STANDARD OPERATING PROCEDURE

HAZARDOUS CHEMICALS/CLASS OF HAZARDOUS CHEMICALS

• Flammable liquids are chemicals that have a flash point below 100^oF (38.7^o C) and a vapor pressure that does not exceed 40 psig at 100^oF.

HAZARD DESCRIPTION

• Contact with flame or hot surfaces will result in a fire.

PROTECTION PROCESS

• Good laboratory technique
• Appropriate shielding through use of personal protective equipment

• Experiments involving greater than 500 mL of flammable liquids should be carried out in a fume hood.

PERSONAL PROTECTIVE EQUIPMENT

• Eye protection in the form of safety glasses must be worn at all times when handling flammable liquids. Ordinary (street) prescription glasses do not provide adequate protection.

• Gloves should be worn when handling flammable liquids. Disposable latex or nitrile gloves provide adequate protection against accidental hand contact with small quantities of most laboratory chemicals. Lab workers should contact OEHS for advice on chemical resistant glove selection when direct or prolonged contact with hazardous chemicals is anticipated.

• Lab coats, closed toed shoes and long sleeved clothing should be worn when handling flammable liquids. Additional protective clothing should be worn if the possibility of skin contact is likely.

• Safety shielding is required any time there is a risk of explosion, splash hazard or a highly exothermic reaction. All manipulations of flammable liquids which pose this risk should occur in a fume hood with the sash in the lowest feasible position. Safety shielding is required any time there is a risk of explosion, splash hazard or a highly exothermic reaction. Portable shields, which provide protection to all laboratory occupants, are acceptable.

ENGINEERING/VENTILATION CONTROLS

• A safety shower and eyewash must be available and accessible when working with corrosive liquids. Bottle-type eyewash stations are not acceptable.

• A fume hood should be used when handling volumes of flammable liquids larger than 500 mL.

• Manipulation of flammable liquids outside of a fume hood may require special ventilation controls in order to minimize exposure to the material. Fume hoods provide the best protection against exposure to flammable liquids in the laboratory and are the preferred ventilation control device. Always attempt to handle large quantities of flammable liquids in a fume hood. If your research does not permit the handing of large quantities of flammable liquids in your fume hood, contact the Office of Environmental Health and Safety to review the adequacy of all special ventilation.

SPECIAL HANDLING PROCEDURES AND STORAGE REQUIREMENTS

• The storage of flammable and combustible liquids in a laboratory, shop or building area must be kept to the minimum needed for research and/or operations. If more than 5 gallons of flammables are present outside of safety cans per 100 square feet of area, a flammable-liquids storage cabinet is required. Flammable-liquids storage cabinets are not intended for the storage of highly toxic materials, acids, bases, compressed gases or pyrolytic chemicals.

• All flammable liquids must be clearly labelled with the correct chemical name. Handwritten labels are acceptable; chemical formulas and structural formulas are not acceptable.

• Where feasible (if the quality of the solvent will not be adversely affected) transfer flammable liquids from glass bottles into metal safety cans.

• Mechanical vacuum pumps must be protected using cold traps and, where appropriate, filtered to prevent particulate release. The exhaust for the pumps must be vented into an exhaust hood. Vacuum pumps should be rated for use with flammable liquids.

SPILL AND ACCIDENT PROCEDURES

• Anticipate spills by having the appropriate clean up equipment on hand. The appropriate clean up supplies can be determined by consulting the material safety data sheet. This should occur prior to the use of any flammable liquids. Spill supplies for flammable liquids are designed to control the liquid portion of the spill and minimize the production of flammable vapors. Never use paper towels on large spills of flammable liquids because it exacerbates vapor production.

• In the event of a spill all personnel in the area should be alerted. Turn off all sources of ignition. Do not attempt to handle a large spill of flammable liquids. Vacate the laboratory immediately and call for assistance: Office of Environmental Health & Safety 292-1284 or 911 (This is a 24 hour service).

• Remain on the scene, but at a safe distance, to receive and direct safety personnel when they arrive.

WASTE DISPOSAL

• Collect waste in an approved safety container and record date, chemical, and amount.

• Call Office of Environmental health & Safety (292-1284) for pickup.

SPECIAL APPROVAL REQUIRED

• No

DECONTAMINATION

• Wash hands and arms with soap and water immediately following any skin contact with flammable liquids.

DESIGNATED AREA

• No

 

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FORMALDEHYDE
STANDARD OPERATING PROCEDURE

HAZARDOUS CHEMICALS/CLASS OF HAZARDOUS CHEMICALS

• Formaldehyde (37%) or Formalin

HAZARD DESCRIPTION

• Regulated Carcinogen. Combustible Liquid, Corrosive, Sensitizer

PROTECTION PROCESS

• Good laboratory technique
• Skin and eye protection should be used when working with formaldehyde
• If airborne exposures are suspected, contact EHS (292-1284) for consultation

PERSONAL PROTECTIVE EQUIPMENT

• Eye protection should be selected on potential for splash and exposure. Safety glasses with side shields are sufficient when only low splash hazard exist (eg. placing a tissue sample in a container). Goggles should be worn if using or transferring larger quantities.
• Disposable or lightweight nitrile, neoprene, natural rubber or PVC gloves provide protection from incidental contact. Heavier gloves should be used when extended handling of contaminated or preserved materials or immersion is likely.
• A chemically resistant apron should be used when transferring or using large quantities and splash is likely.

ENGINEERING/VENTILATION CONTROLS

• Work with formaldehyde in a fume hood or with or local exhaust ventilation.
• Use only in an area equipped with a safety shower and eyewash.

SPECIAL HANDLING PROCEDURES AND STORAGE REQUIREMENTS

• Keep in a tightly closed container.
• Separate from oxidizing agents.
• Whenever formaldehyde is mixed and stored outside its original container, it must be clearly marked with its concentration and a description of its hazards.

SPILL AND ACCIDENT PROCEDURES

• Absorb incidental small spills. Collect and submit for waste disposal by EHS. (Do not attempt cleanup if you feel unsure of your ability to do so or if you perceive the risk to be greater than normal laboratory operations.
• In case of large spills, notify others in area, and evacuate room/immediate area. Call EHS 292-1284 for hazmat response. Post area and prevent unnecessary entry until EHS response team arrives. Provide assistance to EHS as requested.
• If splashed on an individual or in eyes flush for 15 minutes with copious quantities of water.

WASTE DISPOSAL

• Dispose of waste through OSU-EHS (292-1284).

SPECIAL APPROVAL REQUIRED

• Recommended.

DECONTAMINATION

• Rinse exposed area of any skin contact with copious amounts of water.

DESIGNATED AREA

• Recommended.

 

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OXIDIZING CHEMICALS
STANDARD OPERATING PROCEDURE

HAZARDOUS CHEMICALS/CLASS OF HAZARDOUS CHEMICALS

• Oxidizing chemicals are materials that spontaneously evolve oxygen at room temperature or with slight heating or promote combustion.
• This class of chemicals includes peroxides, chlorates, perchlorates, nitrates, and permanganates.
• Examples of strong oxidizers are listed at the end of this SOP.

HAZARD DESCRIPTION

• Strong oxidizers are capable of forming explosive mixtures when mixed with combustible, organic or easily oxidized materials

PROTECTION PROCESS

• Good laboratory technique
• Appropriate shielding through use of personal protective equipment
• Use of fume hood
• Availability of eye wash station and safety shower

PERSONAL PROTECTIVE EQUIPMENT

• Safety glasses/goggles (Wear chemical safety goggles when using small quantities or safety glasses or chemical safety goggles with face shield when using large quantities or when a splash potential exists.)
• Gloves should be worn when handling oxidizing chemicals. Disposable latex or nitrile gloves provide adequate protection against accidental hand contact with small quantities of most laboratory chemicals. Lab workers should contact OEHS for advice on chemical resistant gloves when direct or prolonged contact with hazardous chemicals is anticipated.
• Lab coats, closed toed shoes and long sleeved clothing should be worn when handling oxidizing chemicals.
• Additional protective clothing should be worn if the possibility of skin contact is likely.

ENGINEERING/VENTILATION CONTROLS

• The use of certain concentrations of perchloric acid must be performed in a fume hood equipped with wash down facilities. Contact the Office of Environmental Health and Safety for fume hood requirements.
• Safety shielding is required any time there is a risk of explosion, splash hazard or a highly exothermic reaction. All manipulations of oxidizing chemicals which pose this risk should occur in a fume hood with the sash in the lowest feasible position. Portable shields, which provide protection to all laboratory occupants are acceptable.
• A safety shower and eyewash must be available and accessible when working with acutely toxic chemicals.

SPECIAL HANDLING PROCEDURES AND STORAGE REQUIREMENTS

• Oxidizers should be stored in a cool and dry location.
• Keep oxidizers segregated from all other chemicals in the laboratory.
• Minimize the quantities of strong oxidizers stored in the laboratory.

• Never return excess chemicals to the original container. Small amounts of impurities may be introduced into the container which may cause a fire or explosion.

SPILL AND ACCIDENT PROCEDURES

• Before beginning work with oxidizing chemicals, develop emergency procedures which address response actions to fires, explosions, spills, injury to staff, or the development of signs and symptoms of overexposure. The procedures should address as a minimum the following:

• Who to contact: (University police, and Office of Environmental Health and Safety,
• Principal investigator of the laboratory including evening phone number)
• The location of all safety equipment (showers, spill clean up supplies, eye wash, fire extinguishers, etc.)
• The method used to alert personnel in nearby areas of potential hazards
• Special first aid treatment required by the type of corrosive material(s) handled in the laboratory

• Anticipate spills by having clean up equipment on hand. The appropriate clean up supplies can be determined by consulting the material safety data sheet. This should occur prior to the use of any oxidizing chemicals.
• Spill control materials for oxidizers are designed to be inert and will not react with the reagent.
• Never use paper towels or other inappropriate materials which are combustible.
• The waste materials generated during spill cleanup may pose a flammability risk and should not remain in the laboratory overnight unless it is stored in an appropriate container.

• In the event of a spill, all personnel in the area should be alerted.
• Do not attempt to handle a large spill of oxidizing chemicals. Vacate the laboratory immediately and call for assistance (Division of Environmental Health & Safety 292-1284 or 911).
• Remain on the scene, but at a safe distance, to receive and direct safety personnel when they arrive.

WASTE DISPOSAL

• All materials contaminated with oxidizing chemicals pose a fire hazard and should be disposed of as hazardous waste.
• Alert the Office of Environmental Health and Safety if you generate wastes contaminated by oxidizers.
• Do not let contaminated wastes remain in the laboratory overnight unless proper containers are provided.

SPECIAL APPROVAL REQUIRED

• You should notify the Office of Environmental Health and Safety prior to the initial use of perchloric acid.

DECONTAMINATION

• Personnel: Wash hands and arms with soap and water immediately after handling oxidizing chemicals.

• Area: Carefully clean work area after use. Paper towels or similar materials contaminated with strong oxidizing chemicals may pose a fire risk.

DESIGNATED AREA

• No

EXAMPLES OF STRONG OXIDIZERS

• Ammonium perchlorate
• Ammonium permanganate
• Barium peroxide
• Bromine
• Calcium chlorate
• Calcium hypochlorite
• Chlorine trifluoride
• Chromium anhydride
• Chromic acid
• Dibenzoyl peroxide
• Fluorine
• Hydrogen peroxide
• Mangesium peroxide
• Nitrogen trioxide
• Perchloric acid
• Potassium bromate
• Potassium chlorate
• Potassium peroxide
• Propyl nitrate
• Sodium chlorate
• Sodium chlorite
• Sodium perchlorate
• Sodium peroxide

Source: CRC Handbook of Laboratory Safety, 3rd edition.

 

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PYROPHORIC CHEMICALS
STANDARD OPERATING PROCEDURE

HAZARDOUS CHEMICALS/CLASS OF HAZARDOUS CHEMICALS

• Pyrophoric chemicals are liquids and solids that will ignite spontaneously in air at about 130° F. A listing of some pyrophoric chemicals is at the end of this SOP.

HAZARD DESCRIPTION

• Pyrophoric chemicals can explode or produce a fire.

PROTECTION PROCESS

• Good laboratory technique
• Appropriate shielding through use of personal protective equipment
• Use of fume hood
• Availability of eye wash station and safety shower

PERSONAL PROTECTIVE EQUIPMENT

• Safety glasses/goggles (Wear chemical safety goggles when using small quantities or safety glasses or chemical safety goggles with face shield when using large quantities or when a splash potential exists.)
• Gloves should be worn when handling pyrophoric chemicals. Disposable latex or nitrile gloves provide adequate protection against accidental hand contact with small quantities of most laboratory chemicals. Lab workers should contact OEHS for advice on chemical resistant gloves when direct or prolonged contact with hazardous chemicals is anticipated.
• Lab coats, closed toed shoes and long sleeved clothing should be worn when handling pyrophoric chemicals.
• Additional protective clothing should be worn if the possibility of skin contact is likely.

ENGINEERING/VENTILATION CONTROLS

• Many pyrophoric chemicals release noxious or flammable gases and should be handled in a hood.

• Some pyrophoric materials are stored under kerosene (or other flammable solvents), therefore the use of a fume hood is required to prevent the release of flammable vapors in the laboratory.
• Glove boxes may be used to handle pyrophoric chemicals if inert or dry atmospheres are required.

SPECIAL HANDLING PROCEDURES AND STORAGE REQUIREMENTS

• All pyrophoric chemicals must be clearly labelled with the correct chemical name.
• Pyrophoric chemicals should be stored under an atmosphere of inert gas or under kerosene as appropriate.
• Do not store pyrophoric chemicals with flammable materials or in a flammable-liquids storage cabinet.
• Store these materials away from sources of ignition. Minimize the quantities of pyrophoric chemicals stored in the laboratory.
• Never return excess chemicals to the original container. Small amounts of impurities may be introduced into the container which may cause a fire or explosion.

SPILL AND ACCIDENT PROCEDURES

• Before beginning work with pyrophoric chemicals, develop emergency procedures which address response actions to accidental exposure from fires, explosions, or spills. The procedures should address as a minimum the following:

• Who to contact: (University police, and Office of Environmental Health and Safety,
• Principal investigator of the laboratory including evening phone number)
• The location of all safety equipment (showers, spill clean up supplies, eye wash, fire extinguishers, etc.)
• The method used to alert personnel in nearby areas of potential hazards
• Special spill control materials required by the type of corrosive material(s) handled in the laboratory

• Anticipate spills by having clean up equipment on hand. The appropriate clean up supplies can be determined by consulting the material safety data sheet. This should occur prior to the use of any pyrophoric chemicals.

• In the event of a spill, all personnel in the area should be alerted.
• Do not attempt to handle a large spill of pyrophoric chemicals. Vacate the laboratory immediately and call for assistance (Division of Environmental Health & Safety 292-1284 or 911).
• Remain on the scene, but at a safe distance, to receive and direct safety personnel when they arrive.

WASTE DISPOSAL

• All materials contaminated with pyrophoric chemicals should be disposed of as a hazardous waste.
• Alert the Office of Environmental Health and Safety if you generate wastes contaminated with pyrophoric chemicals. These wastes may pose a flammability risk and should not remain in the laboratory overnight.

SPECIAL APPROVAL REQUIRED

• No.

DECONTAMINATION

• Wash hands and arms with soap and water immediately following any skin contact with pyrophoric chemicals.

DESIGNATED AREA

• No.

EXAMPLES OF PYROPHORIC COMPOUNDS

Grignard reagents, RMgX

Metal alkyls and aryls, such as RLi, RNa, R3Al, R2Zn

Metal carbonyls, such as Ni(CO)4, Fe(CO)5, Co2(CO)8

Alkali metals such as Na, K

Metal powders, such as Al, Co, Fe, Mg, Mn, Pd, Pt, Ti, Sn, Zn, Zr

Metal hydrides, such as NaH, LiAlH4

Nonmetal hydrides, such as B2H6 and other boranes, PH3, AsH3

Nonmetal alkyls, such as R3B, R3P, R3As

Phosphorus (white)

 

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REPRODUCTIVE TOXINS
STANDARD OPERATING PROCEDURE

HAZARDOUS CHEMICALS/CLASS OF HAZARDOUS CHEMICALS

• Reproductive hazards are substances which affect the reproductive capabilities including chromosomal damage (mutagens) and effects on the fetus (teratogens). A list of some reproductive hazards is included at the end of this SOP.

HAZARD DESCRIPTION

• Reproductive hazards can cause birth defects.

PROTECTION PROCESS

• Good laboratory technique
• Appropriate shielding through use of personal protective equipment
• Use of fume hood
• Availability of eye wash station and safety shower

PERSONAL PROTECTIVE EQUIPMENT

• Safety glasses/goggles (Wear chemical safety goggles when using small quantities or safety glasses or chemical safety goggles with face shield when using large quantities or when a splash potential exists.)
• Gloves should be worn when handling reproductive toxins. Disposable latex or nitrile gloves provide adequate protection against accidental hand contact with small quantities of most laboratory chemicals. Lab workers should contact OEHS for advice on chemical resistant gloves when direct or prolonged contact with hazardous chemicals is anticipated.
• Lab coats, closed toed shoes and long sleeved clothing should be worn when handling oxidizing chemicals.
• Additional protective clothing should be worn if the possibility of skin contact is likely.

ENGINEERING/VENTILATION CONTROLS

• Manipulation of reproductive hazards should be carried out in a fume hood.
• Under certain conditions, reproductive toxins can be used in a biological safety cabinet. The biological safety cabinet is designed to remove the reproductive toxins before the air is discharged into the environment. Reproductive toxins that are volatile must not be used in a biological safety cabinet unless the cabinet is vented to the outdoors.
• Certain reproductive hazards must be handled in a glove box rather than a fume hood. The Division of Environmental Health and Safety or the Principal Investigator will determine if this is required.
• For small quantities of reproductive toxins, portable shields, which provide protection to all laboratory occupants, are acceptable.
• A safety shower and eyewash must be available and accessible when working with reproductive toxins.

SPECIAL HANDLING PROCEDURES AND STORAGE REQUIREMENTS

• Reproductive hazards must be stored in a designated area.

SPILL AND ACCIDENT PROCEDURES

• Before beginning work with reproductive toxins, develop emergency procedures which address response actions to accidental exposure from fires, explosions, or spills. The procedures should address as a minimum the following:

• Who to contact: (University police, and Office of Environmental Health and Safety,
• Principal investigator of the laboratory including evening phone number)
• The location of all safety equipment (showers, spill clean up supplies, eye wash, fire extinguishers, etc.)
• The method used to alert personnel in nearby areas of potential hazards
• Special first aid treatment required by the type of corrosive material(s) handled in the laboratory

• Anticipate spills by having clean up equipment on hand. The appropriate clean up supplies can be determined by consulting the material safety data sheet. This should occur prior to the use of any reproductive toxin.

• In the event of a spill, all personnel in the area should be alerted.
• Do not attempt to handle a large spill of reproductive toxins. Vacate the laboratory immediately and call for assistance (Division of Environmental Health & Safety 292-1284 or 911).
• Remain on the scene, but at a safe distance, to receive and direct safety personnel when they arrive.

WASTE DISPOSAL

• All materials contaminated with reproductive hazards should be disposed of as a hazardous waste.
• Wherever possible, attempt to design research in a manner that reduces the quantity of waste generated.
• Questions regarding waste pick up should be directed to the Office of Environmental Health and Safety.
• This office can also assist you in minimizing waste generation.

SPECIAL APPROVAL REQUIRED

• You should notify the Office of Environmental Health and Safety prior to the initial use of reproductive hazards.
• Notification is also required following significant changes in procedures or the quantity of materials used.

DECONTAMINATION

Personnel:

• Wash hands and arms with soap and water immediately after handling reproductive toxins.

Area:

• Decontamination procedures vary depending on the material being handled. The toxicity of some materials can be neutralized with other reagents. All surfaces should be wiped with the appropriate cleaning agent following dispensing or handling.
• Waste materials generated should be treated as hazardous waste.

Equipment:

• Decontaminate glassware or equipment before removing them from the designated area.

DESIGNATED AREA

• All locations within the laboratory where reproductive hazards are handled should be demarcated with designated area caution tape (available from OHES 292-1284) and/or posted with designated area caution signs. This includes all fume hoods and bench tops where the reproductive hazards are handled.
• Where feasible, reproductive hazards should be manipulated over plastic-backed disposable paper work surfaces. These disposable work surfaces minimize work area contamination and simplify clean up.
• The room sign for the laboratory must contain a "Designated Areas Within" identifier.

EXAMPLES OF REPRODUCTIVE TOXINS

• Acrylic acid
• Aniline
• Benzene
• Cadmium
• Carbon disulfide
• N,N-dimethylacetamide
• Dimethylformamide (DMF)
• Dimethyl sulfoxide (DMSO)
• Diphenylamine
• Estradiol
• Formaldehyde
• Formamide
• Hexachlorobenzene
• Iodoacetic acid
• Lead compounds Mercury compounds
• Nitrobenzene
• Nitrous oxide
• Phenol
• Polychlorinated and polybrominated biphenyls
• Toluene
• Vinyl chloride
• Xylene

 

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WATER REACTIVE CHEMICALS
STANDARD OPERATING PROCEDURE

HAZARDOUS CHEMICALS/CLASS OF HAZARDOUS CHEMICALS

• Water sensitive chemicals are chemicals that react vigorously with moisture.
• The most common water sensitive chemicals include sodium, potassium, lithium metals and aluminum alkyls. A list of some water reactive chemicals is at the end of this SOP.

HAZARD DESCRIPTION

• Water reactive chemicals can react explosively when they come into contact with water.

PROTECTION PROCESS

• Good laboratory technique
• Appropriate shielding through use of personal protective equipment
• Use of fume hood
• Availability of eye wash station and safety shower

PERSONAL PROTECTIVE EQUIPMENT

• Safety glasses/goggles (Wear chemical safety goggles when using small quantities or safety glasses or chemical safety goggles with face shield when using large quantities or when a splash potential exists.)
• Gloves should be worn when handling water reactive chemicals. Disposable latex or nitrile gloves provide adequate protection against accidental hand contact with small quantities of most laboratory chemicals. Lab workers should contact OEHS for advice on chemical resistant gloves when direct or prolonged contact with hazardous chemicals is anticipated.
• Lab coats, closed toed shoes and long sleeved clothing should be worn when handling water reactive chemicals.
• Additional protective clothing should be worn if the possibility of skin contact is likely.

ENGINEERING/VENTILATION CONTROLS

• Many water sensitive chemicals will liberate hydrogen when they react with water. The use of a fume hood is recommended to prevent the buildup of combustible gases.
• A glove box may be used to handle water sensitive chemicals when a dry atmosphere is required.

• A safety shower and eyewash must be available and accessible when working with water reactive chemicals.

SPECIAL HANDLING PROCEDURES AND STORAGE REQUIREMENTS

• Special ventilation is required if these materials are used outside of a fume hood. If your research does not permit the handing of water sensitive chemicals in a fume hood you must contact the Office of Environmental Health and Safety to review the adequacy of all special ventilation.

• Water sensitive chemicals should be stored in a cool and dry location.
• Keep water sensitive chemicals segregated from all other chemicals in the laboratory.
• Minimize the quantities of water sensitive chemicals stored in the laboratory.
• Date all containers upon receipt.
• Potassium will form peroxides and superoxides when stored under oil at room temperature. Examine storage containers frequently.
• Dispose of any container that exhibits salt build up on its exterior.
• Dispose of all water sensitive chemicals whenever they are no longer required for current research.
• Never return excess chemicals to the original container. Small amounts of impurities may be introduced into the container which may cause a fire or explosion.

SPILL AND ACCIDENT PROCEDURES

• Before beginning work with water reactive chemicals, develop emergency procedures which address response actions to accidental exposure from fires, explosions, or spills. The procedures should address as a minimum the following:

• Who to contact: (University police, and Office of Environmental Health and Safety,
• Principal investigator of the laboratory including evening phone number)
• The location of all water reactive chemicals in the laboratory.
• The method used to alert personnel in nearby areas of potential hazards
• Special first aid treatment required by the type of water reactive chemical(s) handled in the laboratory

• Anticipate spills by having clean up equipment on hand. The appropriate clean up supplies can be determined by consulting the material safety data sheet. This should occur prior to the use of any water reactive chemicals.
• Do not put water on the spill.
• Spill control materials for water sensitive chemicals are designed to be inert and will not react with the reagent.

• In the event of a spill, all personnel in the area should be alerted.
• Do not attempt to handle a large spill of water reactive chemicals. Turn off all ignition sources and vacate the laboratory immediately and call for assistance (Division of Environmental Health & Safety 292-1284 or 911).
• Remain on the scene, but at a safe distance, to receive and direct safety personnel when they arrive.

WASTE DISPOSAL

• All materials contaminated with water sensitive chemicals should be disposed of as hazardous waste.
• Alert the Office of Environmental Health and Safety if you generate wastes contaminated by water sensitive chemicals.
• These wastes should not remain in the laboratory overnight as they may pose a flammability risk.

SPECIAL APPROVAL REQUIRED

• No.

DECONTAMINATION

Personnel:

• Wash hands and arms with soap and water immediately after handling water sensitive materials.

Area:

• Carefully clean work area after use.

DESIGNATED AREA

• No.

EXAMPLES OF WATER REACTIVE CHEMICALS

• Alkali metals, such as Na, Li, K
• Alkali metal hydrides, such as LiH, CaH2, LiAlH4, NaBH4, alkali metal amides, such as NaNH2
• Metal alkyls, such as lithium and aluminum alkyls
• Grignard reagents, RMgX
• Halides of nonmetals, such as BCl3, BF3, PCl3, PCl5, SiCl4, S2Cl2
• Inorganic acid halides, such as POCl3, SOCl2, SO2Cl2
• Anhydrous metal halides, such as AlCl3, TiCl4, ZrCl4, SnCl4
• Phosphorus pentoxide
• Calcium carbide
• Organic acid halides and anhydrides of low molecular weight, such as, acetylchloride acetic acid nhydride

 

 

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