Education
- Postdoctoral Fellowships: Biological Chemistry Department of John Innes Center, England, UK; Biochemistry Department of the Dundee University, Scotland, UK and HIV Drug Resistance Program of the National Cancer Institute, MD, USA.
- Ph.D. Biochemistry (Chemical Enzymology) 1990, The Georgian Institute and the Biotechnology Center of the Moscow State University, Russia;
Areas of Interest
The focus of our research is to understand HIV replication, cancer development and DNA repair processes at the molecular level. The knowledge obtained will be exploited to develop new, highly effective anti-viral and anti-cancer drugs. We primarily use mass spectrometry as both a proteomic and structural biology tool to elucidate the composition and architecture of key biological macromolecules. In particular, powerful mass spectrometric methodologies are being developed to obtain high-resolution structural information on protein-nucleic acid, protein-protein and drug-protein complexes. Proteomic applications in our laboratory include dissection of human proteins participating in HIV replication, revealing essential posttranslational modifications in proteins, discovery of biomarkers for the early detection of cancer and identification of factors responsible for anti-cancer drug resistance. We augment our structural and proteomic studies with traditional biochemical, molecular biology and biophysical analysis to obtain a comprehensive picture of these important biological events.
Recent Publications
Shkriabai N, Datta SA, Zhao Z, Hess S, Rein A, Kvaratskhelia M (2006) HIV-1 Gag interactions with assembly cofactors. Biochemistry 45: 4077-83.
Liu Y , Kvaratskhelia M , Hess S , Qu Y , Zou Y . (2005) Modulation of replication protein A function by its hyperphosphorylation-induced conformational change involving DNA binding domain B. J. Biol. Chem. 280:32775-83.
Williams KL, Zhang Y, Shkriabai N, Karki RG, Nicklaus MC, Kotrikadze N, Hess S, Le Grice SF, Craigie R, Pathak VK, Kvaratskhelia M. (2005) Mass spectrometric analysis of the HIV-1 integrase-pyridoxal 5'-phosphate complex reveals a new binding site for a nucleotide inhibitor. J. Biol. Chem. 280:7949-55.
Shell SM, Hess S, Kvaratskhelia M, Zou Y. (2005) Mass spectrometric identification of lysines involved in the interaction of human replication protein A with single-stranded DNA. Biochemistry. 25:971-8.
Shkriabai N, Patil SS, Hess S, Budihas SR, Craigie R, Burke TR, Le Grice SF, Kvaratskhelia M. (2004) dentification of an inhibitor-binding site to HIV-1 integrase with affinity acetylation and mass spectrometry.Proc. Natl. Acad. Sci. U S A. 101:6894-9.
Kvaratskhelia M , Clark PK, Hess S, Melder DC, Federspiel MJ, Hughes SH. (2004) Identification of glycosylation sites in the SU component of the Avian Sarcoma/Leukosis virus Envelope Glycoprotein (Subgroup A) by mass spectrometry. Virology. 326:171-81.
Lener D, Kvaratskhelia M, Le Grice SF. (2003) Nonpolar thymine isosteres in the Ty3 polypurine tract DNA template modulate processing and provide a model for its recognition by Ty3 reverse transcriptase. J. Biol. Chem. 278: 26526-32.
Kvaratskhelia M, Miller JT, Budihas SR, Pannell LK, Le Grice SFJ. (2002) Identification of Specific HIV-1 Reverse Transcriptase Contacts to the Viral RNA:tRNA Complex by Mass Spectrometry and a Primary Amine Selective Reagent. Proc. Natl. Acad. Sci. USA. 99: 15988-93.
Kvaratskhelia M, Budihas SR, Le Grice SFJ. (2002) Pre-existing Distortions in Nucleic Acid Structure Aid Polypurine Tract Selection by HIV-1 Reverse Transcriptase. J. Biol. Chem. 277: 16689-96.
Kvaratskhelia M, Wardleworth BN, Bond CS, Fogg JM, Lilley DMJ, White MF. (2002) Holliday junction resolution is modulated by archaeal chromatin components in vitro. J. Biol. Chem. 277: 2992-6.
Fogg JM, Kvaratskhelia M, White MF, Lilley DMJ. (2001) Distortion of DNA junctions imposed by the binding of resolving enzymes: A fluorescence study. J. Mol. Biol.313: 751-64.
Bond CS, Kvaratskhelia M, Richard D, White MF, Hunter WN. (2001) Structure of Hjc, a Holliday junction resolvase, from Sulfolobus solfataricus. Proc. Natl. Acad. Sci. USA. 98: 5509-14.
Napoli A, Kvaratskhelia M, White MF, Rossi M, Ciaramella M. (2001) A novel member of the bacterial-archaeal regulator family is a nonspecific DNA-binding protein and induces positive supercoiling. J. Biol. Chem. 276: 10745-52.
Kvaratskhelia M, Wardleworth BN, Norman DG, White MF. (2000) A conserved nuclease domain in the archaeal Holliday junction resolving enzyme Hjc. J. Biol. Chem. 275: 25540-6.
Wardleworth BN, Kvaratskhelia M, White MF. (2000) Site-directed mutagenesis of the yeast resolving enzyme Cce1 reveals catalytic residues and relationship with the intron-splicing factor Mrs1. J. Biol. Chem. 275: 23725-8.
Kvaratskhelia M, White MF. (2000) Two Holliday junction resolving enzymes in Sulfolobus solfataricus. J. Mol. Biol. 297: 923-32.
Kvaratskhelia M, White MF. (2000) An archaeal Holliday junction resolving enzyme from Sulfolobus solfataricus exhibits unique properties. J. Mol. Biol. 295: 193-202.
Kvaratskhelia M, George SJ, Cooper A, White MF. (1999) Quantitation of metal ion and DNA junction binding to the Holliday junction endonuclease Cce1. Biochemistry. 38: 16613-9.
Graduate Courses Taught
Pharmacy 801: Biomedical Proteomics and Mass Spectrometry
Presentation of some basic principles and techniques used in pharmaceutical research and product development: drug stabilization, solubilization, complexation, and macromolecule interactions.
Pharmacy 850.01: Presentation
Presentation of oral and written reports dealing with recent advances in the pharmaceutical sciences.
Professional Experience
Associate Professor, The Ohio State University, College of Pharmacy, Center for Retrovirus Research and Comprehensive Cancer Center, Columbus, OH.
Assistant Professor, The Ohio State University, College of Pharmacy, Center for Retrovirus Research and Comprehensive Cancer Center, Columbus, OH.
[ back to top ]
|
